Indirect immunostaining of PFA fixed rat hippocampus neurons (dilution 1 : 500; red). Nuclei have been visualized by DAPI staining (blue).

Indirect immunolabeling with anti-calbindin (dilution 1 : 200; red) and guinea pig anti-calbindin D28k (cat. no. 214 004, dilution 1 : 200; green) of a PFA fixed mouse cortex section. Immunoreactive structures have been revealed with fluorochromated secondary antibodies. Nuclei have been visualized by DAPI staining.

Indirect immunostaining of PFA fixed paraffin embedded mouse hippocampus section (dilution 1 : 200) using DAB as a chromogen. Nuclei have been visualized by haematoxylin staining (blue).